Regulatory

Part:BBa_K112118:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


rrnB P1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

PCR mv028/mv029 on rrnB native sequence   (721bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                         (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112118
-----------------------------------------------
mv028	Forward Biobricking of rrnB P1 promoter
cgataGAATTCatgAGATCTatgcactaaaacgtatcctacgcccg
mv029   Reverse Biobricking of rrnB P1 promoter
cgttaGGATCCcgccttcccgctacagagtcaagc

References